CCL2 and CCL5 driven attraction of CD172a + monocytic cells during an equine herpesvirus type 1 (EHV-1) infection in equine nasal mucosa and the impact of two migration inhibitors, rosiglitazone (RSG) and quinacrine (QC)
AbstractEquine herpesvirus type 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Besides epithelial cells, CD172a+ monocytic cells become infected with EHV-1 in the respiratory mucosa and transport the virus from the apical side of the epithelium to the lamina propria en route to the lymph and blood circulation. Whether CD172a+ monocytic cells are specifically recruited to the infection sites in order to pick up virus is unknown. In our study, equine nasal mucosa explants were inoculated with EHV-1 neurological strains 03P37 and 95P105 or the non-neurological strains 97P70 and 94P247 and the migration of monocytic cells was examined by immunofluorescence. Further, the role of monokines CCL2 and CCL5 was determined and the effect of migration inhibitors rosiglitazone (RSG) or quinacrine was analyzed. It was shown that with neurological strains but not with the non-neurological strains, CD172a+ cells specifically migrated towards EHV-1 infected regions and that CCL2 and CCL5 were involved. CCL2 started to be expressed in infected epithelial cells at 24 h post-incubation (hpi) and CCL5 at 48 hpi, which corresponded with the CD172a+ migration. RSG treatment of EHV-1-inoculated equine nasal mucosa had no effect on the virus replication in the epithelium, but decreased the migration of CD172a+ cells in the lamina propria. Overall, these findings bring new insights in the early pathogenesis of EHV-1 infections, illustrate differences be...
CORRIGENDUM FOR "Reductions in Insulin Resistance are Mediated Primarily via Weight Loss in Subjects With Type 2 Diabetes on Semaglutide". J Clin Endocrinol Metab. 2020 Jan 01;105(1): Authors: PMID: 31832679 [PubMed - in process]
ERRATUM FOR "Genetic Link Between Gender Dysphoria and Sex Hormone Signaling". J Clin Endocrinol Metab. 2020 Jan 01;105(1): Authors: PMID: 31832678 [PubMed - in process]
CORRIGENDUM FOR "Role of Activin-A and Myostatin and Their Signaling Pathway in Human Myometrial and Leiomyoma Cell Function". J Clin Endocrinol Metab. 2020 Jan 01;105(1): Authors: PMID: 31832677 [PubMed - in process]
CORRIGENDUM for "Dietary Calcium Intake and Bone Loss Over 6 Years in Osteopenic Postmenopausal Women". J Clin Endocrinol Metab. 2020 Jan 01;105(1): Authors: PMID: 31832676 [PubMed - in process]
ERRATUM FOR "Serum Lipids in Association With Type 2 Diabetes Risk and Prevalence in a Chinese Population". J Clin Endocrinol Metab. 2020 Jan 01;105(1): Authors: PMID: 31832675 [PubMed - in process]
CORRIGENDUM FOR "The Effect of Testosterone on Cardiovascular Biomarkers in the Testosterone Trials". J Clin Endocrinol Metab. 2020 Jan 01;105(1): Authors: PMID: 31832674 [PubMed - in process]
ERRATUM FOR "Insights Into Local Orbital Immunity: Evidence for the Involvement of the Th17 Cell Pathway in Thyroid-Associated Ophthalmopathy". J Clin Endocrinol Metab. 2020 Jan 01;105(1): Authors: PMID: 31832673 [PubMed - in process]
CORRIGENDUM FOR "Lipid Profiling of Peri-implantation Endometrium in Patients With Premature Progesterone Rise in the Late Follicular Phase". J Clin Endocrinol Metab. 2020 Jan 01;105(1): Authors: PMID: 31832672 [PubMed - in process]
Abstract Hepatitis B surface antigen (HBsAg) level plays an important role in conjunction with other markers such as hepatitis B envelope antigen (HBeAg) and hepatitis B virus (HBV) deoxyribonucleic acid levels to predict disease activity in chronic Hepatitis B (CHB). Quantification of HBsAg is useful in differentiating carriers from active hepatitis in HBeAg negative patients, and current guidelines recommend monitoring of pegylated interferon alpha treatment in CHB infection. However, there are only few studies about the role of quantitative HBsAg (qHBsAg) monitoring in HIV-HBV coinfected patients. Studies have ...
Abstract In recent years, there have been numerous calls by researchers to adopt multi-disciplinary and international perspectives to address the HIV pandemic. Meaningful and prudent public health policy should be based on sound empirical data and research. Henceforth, our study aims to contribute to the current literature by conducting a comprehensive global mapping and determine the landscapes of HIV/AIDS research covering the years between 1983 and 2017. Bibliometric and content analysis was used to describe trends in research productivity, usages, research collaborations, and clusters of research topics. Explo...