Overexpression of phosphodiesterase-4 subtypes involved in surgery-induced neuroinflammatory cognitive dysfunction in mice.

Overexpression of phosphodiesterase-4 subtypes involved in surgery-induced neuroinflammatory cognitive dysfunction in mice. Brain Res Bull. 2017 Feb 21;: Authors: Wang W, Fu Q, Zhang XY, Feng ZG, Zhang H, Sui B, Zhang YY, Zhao WX, Xu ZP, Mi WD Abstract Postoperative cognitive dysfunction (POCD) is characterized by cognitive impairments in patients after surgery. Hippocampal neuroinflammation induced by surgery has been recognized as a pathogenesis of POCD. Phosphodiesterase-4 (PDE4) is an enzyme that specifically hydrolyses cyclic adenosine monophosphate (cAMP), which plays an important role during neuroinflammation and the process of learning and memory. However, the role of PDE4 in the development of POCD remains unclear. Male 14-month-old C57BL/6 mice received carotid artery exposure to mimic POCD. First, we evaluated the cognitive performance by a Morris water maze (MWM) and fear conditioning system (FCS) test after surgery. The expression of PDE4 subtypes, pro-inflammatory cytokines, cAMP, p-CREB and PSD95 were investigated. Then, we used rolipram, a PDE4 inhibitor, to block the effects of PDE4. The cognitive performance of the mice and the expression of PDE4 subtypes, pro-inflammatory cytokines, cAMP, p-CREB and PSD95 were examined again. Mice displayed learning and memory impairment, overexpression of PDE4B and PDE4D, accumulation of pro-inflammatory cytokines, and reduction in the expression of cAMP, p-CREB and PSD95 after su...
Source: Brain Research Bulletin - Category: Neurology Authors: Tags: Brain Res Bull Source Type: research