Bactericidal and fungicidal activity of N-chlorotaurine is enhanced in cystic fibrosis sputum medium.

Bactericidal and fungicidal activity of N-chlorotaurine is enhanced in cystic fibrosis sputum medium. Antimicrob Agents Chemother. 2017 Feb 21;: Authors: Gruber M, Moser I, Nagl M, Lackner M Abstract Lung infections with multiresistant pathogens are a major problem of patients suffering from cystic fibrosis (CF). N-chlorotaurine (NCT), a microbicidal active chlorine compound with no resistance development, is well tolerated upon inhalation. The aim of this study was to investigate NCT on its bactericidal and fungicidal activity in vitro in artificial sputum medium (ASM) mimicking the composition of cystic fibrosis mucus.The medium was inoculated with bacteria (Staphylococcus aureus including some MRSA strains, Pseudomonas aeruginosa, Escherichia coli) or spores of fungi (Aspergillus fumigatus, Aspergillus terreus, Candida albicans, Scedosporium apiospermum, Scedosporium boydii, Lomentospora prolificans, Scedosporium aurantiacum, Scedosporium minutisporum, Exophiala dermatitidis, Geotrichum sp.) to final concentrations of 10(7)-10(8) CFU/ml. NCT was added at 37°C, and time-kill assays were performed.At a concentration of 1% (10 mg/ml, 55 mM) NCT, bacteria and spores were killed within 10 min and 15 min to the detection limit of 10(2) CFU/ml (reduction by 5-6 log10). A reduction by 2 log10 was still achieved by 0.1% (bacteria) and 0.3% NCT (fungi), largely within 10-30 min. Measurements by means of iodometric titration showed oxidizin...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research