The hyperthermophilic cystathionine γ -synthase from the aerobic crenarchaeon Sulfolobus tokodaii: expression, purification, crystallization and structural insights

Cystathionine γ -synthase (CGS; EC 2.5.1.48), a pyridoxal 5 ′ -phosphate (PLP)-dependent enzyme, catalyzes the formation of cystathionine from an l-homoserine derivative and l-cysteine in the first step of the transsulfuration pathway. Recombinant CGS from the thermoacidophilic archaeon Sulfolobus tokodaii (StCGS) was overexpressed in Escherichia coli and purified to homogeneity by heat treatment followed by hydroxyapatite and gel-filtration column chromatography. The purified enzyme shows higher enzymatic activity at 353   K under basic pH conditions compared with that at 293   K. Crystallization trials yielded three crystal forms from different temperature and pH conditions. Form I crystals (space group P21; unit-cell parameters a = 58.4, b = 149.3, c = 90.2   Ã… , β = 108.9 ° ) were obtained at 293   K under acidic pH conditions using 2-methyl-2,4-pentanediol as a precipitant, whereas under basic pH conditions the enzyme crystallized in form II at 293   K (space group C2221; unit-cell parameters a = 117.7, b = 117.8, c = 251.3   Ã… ) and in form II ′ at 313   K (space group C2221; unit-cell parameters a = 107.5, b = 127.7, c = 251.1   Ã… ) using polyethylene glycol 3350 as a precipitant. X-ray diffraction data were collected to 2.2, 2.9 and 2.7   Ã… resolution for forms I, II and II ′ , respectively. Structural analysis of these crystal forms shows that the orientation of the bound PLP in form II is significantly different from that in form II â€...
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: transsulfuration hyperthermophilic enzyme pyridoxal 5 ′ -phosphate methionine biosynthesis Sulfolobus tokodaii cystathionine γ -synthase research communications Source Type: research