Clarithromycin-Based Triple Therapy Is Still Useful as an Initial Treatment for Helicobacter pylori Infection in the Dominican Republic.
Clarithromycin-Based Triple Therapy Is Still Useful as an Initial Treatment for Helicobacter pylori Infection in the Dominican Republic.
Am J Trop Med Hyg. 2017 Feb 13;:
Authors: Miftahussurur M, Cruz M, Subsomwong P, Jiménez Abreu JA, Hosking C, Nagashima H, Akada J, Yamaoka Y
Abstract
Helicobacter pylori antibiotic susceptibility in the Dominican Republic has not been monitored. We assessed Hpylori antibiotic susceptibility in the Dominican Republic, and analyzed Hpylori mutations associated with antibiotic resistance. We recruited 158 dyspeptic patients in Santo Domingo and used agar dilution to test susceptibility to five antibiotics. Polymerase chain reaction-based sequencing was used to assess gyrA, gyrB, rdxA, frxA, and 23S rRNA mutations; next-generation sequencing was used to identify other metronidazole resistance-associated genes. Among 64 Hpylori strains isolated, we identified two (3.1%), one (1.6%), and no strains with clarithromycin, amoxicillin, and tetracycline resistance, respectively. Moreover, high frequency of metronidazole resistance (53/64, 82.8%) was observed, whereas levofloxacin resistance is emerging (23/64, 35.9%). We identified many rdxA and frxA mutations in metronidazole-resistant strains, but no synergistic effect was apparent. We revealed novel mutations in dppA, dppB, fdxA, and fdxB, irrespective of rdxA and frxA mutations. Novel mutations at Ser-14 of trx1 and Arg-221 of dapF were associated with d...
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Miftahussurur M, Cruz M, Subsomwong P, Jiménez Abreu JA, Hosking C, Nagashima H, Akada J, Yamaoka Y Tags: Am J Trop Med Hyg Source Type: research
More News: Amoxicillin | Biaxin | Clarithromycin | Dominican Republic Health | Genetics | Helicobacter Pylori | Levaquin | Metronidazole | Tetracycline | Tropical Medicine