Combination of bladder ultrasonography and novel cystometry method in mice reveals rapid decrease of bladder capacity and compliance in LPS-induced cystitis.

Combination of bladder ultrasonography and novel cystometry method in mice reveals rapid decrease of bladder capacity and compliance in LPS-induced cystitis. Am J Physiol Renal Physiol. 2014 May 7; Authors: Takezawa K, Kondo M, Kiuchi H, Soda T, Takao T, Miyagawa Y, Tsujimura A, Nonomura N, Shimada S Abstract Various animal models have been used in research into bladder dysfunction, and in vivo cystometry is a common method to analyze bladder function in animals. However, it is rather difficult to perform reliably in small animals. Transabdominal bladder ultrasonography combined with cystometry in urethane-anesthetized mice have revealed physical inhibition of bladder wall movement by a bladder catheter conventionally placed in the bladder apex. For reliable evaluation of mouse lower urinary tract function, we established a novel cystometry method in which a catheter was placed in the bladder anterior wall, in combination with bladder ultrasonography. This new method allowed the bladder to be well distended, (i.e., larger maximum bladder capacity, lower pressure threshold, higher voided volume and higher bladder compliance compared with conventional methods), which reflected more spontaneous voiding than conventional cystometry methods. We also demonstrated the usefulness of bladder ultrasonography for analysis of mouse bladder function, especially bladder dynamics, maximum bladder capacity and post-voiding residual volume. We analyz...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research