Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay

Conclusions
To our knowledge, the developed RT-RPA assay is the first sensitive rapid molecular assay applied on field samples for the detection of ZIKV in 15 minutes, which could be implemented at the point of need in a mobile suitcase laboratory 5 to make testing of pregnant women available directly in rural settings. Moreover, combining it with the developed dengue 6, chikungunya 7 and Sigma 8 RT-RPA assays will allow its use during outbreak investigations. Sigma virus infects exclusively dipterans, which is a good candidate to be used in the future as an extraction and RPA reaction control.
Data Availability Statement
All data underlying the findings described in the manuscript is fully available in the body of the manuscript without any restriction.
Conflict of Interest Statement
All authors except Pranav Patel and Olfert Landt are in the public research sector. Mentioned authors are employed by Tib MolBiol, a manufacturer of oligonucleotides. This does not alter the authors´ adherence to all the scientific policies on sharing data and materials.
Corresponding Author
Dr. Ahmed Abd El Wahed, Email: [e-mail] and [e-mail]

Source: PLOS Currents Outbreaks - January 25, 2017 Category: Epidemiology Authors: abdelwahed Source Type: research