D-optimal mixture design optimization of a HPLC method for simultaneous determination of commonly used antihistaminic parent molecules and their active metabolites in human serum and urine.

D-optimal mixture design optimization of a HPLC method for simultaneous determination of commonly used antihistaminic parent molecules and their active metabolites in human serum and urine. Biomed Chromatogr. 2017 Jan 06;: Authors: Kanthiah S, Valliappan K Abstract This present study describes a specific, precise, sensitive and accurate method for simultaneous determination of hydroxyzine, loratadine, terfenadine, rupatadine and their main active metabolites cetirizine, desloratadine and fexofenadine in serum and urine by using meclizine as an internal standard. Solid phase extraction method for sample clean-up and preconcentration of analytes was carried out by using Phenomenex Strata-X-C and Strata X polymeric cartridges. Chromatographic analysis was performed on a Phenomenex cyano (150 mm x 4.6 mm i.d., 5 µm) analytical column. A D-optimal mixture design methodology was used to evaluate the effect of changes in mobile phase compositions on dependent variables and optimization of the response of interest. The mixture design experiments were performed and results were analyzed. The region of ideal mobile phase composition consisting of acetonitrile-methanol-ammonium acetate buffer (40 mM; pH 3.8 adjusted with acetic acid): 18/36/46% v/v/v respectively was identified by a graphical optimization technique using an overlay plot. While using this optimized condition all analytes were baseline resolved in less than 10 min. S...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research