Antimicrobial resistant Escherichia coli and Enterococcus spp. isolated from Miranda Donkey (Equus asinus): an old problem from a new source with a different approach.

Antimicrobial resistant Escherichia coli and Enterococcus spp. isolated from Miranda Donkey (Equus asinus): an old problem from a new source with a different approach. J Med Microbiol. 2017 Jan 05;: Authors: Carvalho I, Del Campo R, Sousa M, Silva N, Carrola J, Marinho C, Santos T, Carvalho S, Nóvoa M, Quaresma M, Pereira JE, Cobo M, Igrejas G, Poeta P Abstract The Miranda Donkey (Equus asinus) is an endangered asinine from Miranda do Douro region, located in North East of Portugal. We study the antimicrobial resistance (AMR) and virulence genes in Escherichia coli and Enterococcus spp. isolates from these animals. In March 2014, a total of 66 faecal samples were recovered from independent animals. Antibiotic resistance was determined by the disk diffusion method. Carriage of genes codifying for antibiotic resistant and virulent factors was analyzed by polymerase chain reaction (PCR). A total of 66 E. coli and 41 enterococci isolates were detected, being the most prevalent species Enterococcus faecium (61%) and Enterococcus hirae (24%). For enterococcal isolates, high percentages of resistant rates to tetracycline (68.3%), quinupristin/dalfopristin (51.2%) and ciprofloxacin (48.8%) were observed. The erm(A) and/or erm(B), tet(M) and/or tet(L), vat(D) and/or vat(E) and aph(3')-IIIa genes were also found. The most frequent virulence gene detected was gel(E), followed by ace, cpd and hyl. E. coli isolates were highly resistant to strep...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research