Lupinifolin from Derris reticulata possesses bactericidal activity on Staphylococcus aureus by disrupting bacterial cell membrane

In this study, lupinifolin, a prenylated flavonoid, was isolated fromDerris reticulata stem, identified by NMR spectra and confirmed with mass spectrometry. Lupinifolin was freshly prepared by solubilizing in 0.1  N NaOH and immediately diluted in Müller–Hinton broth for antibacterial testing. The data showed that Gram-positive bacteria were more susceptible to lupinifolin than Gram-negative bacteria. Of four strains of Gram-positive bacteria tested,Staphylococcus aureus was the most susceptible. Using the two-fold microdilution method, it was found that lupinifolin possessed antimicrobial activity againstS. aureus with minimum inhibitory concentration and minimum bactericidal concentration of 8 and 16  µg/ml, respectively, which is less potent than ampicillin. However, from the time–effect relationship, it was shown that lupinifolin had faster onset than ampicillin. The faster onset of lupinifolin was confirmed by scanning electron microscopy. To investigate the mechanism of action of lupinif olin, transmission electron microscopy (TEM) was performed to observe the ultrastructure ofS. aureus. The TEM images showed that lupinifolin ruptured the bacterial cell membrane and cell wall. Due to its fast onset, it is suggested that the action of lupinifolin is likely to be the direct disruption of the cell membrane. This hypothesis was substantiated by the data from flow cytometry using DiOC2 as an indicator. The result showed that the red/green rat...
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research

Related Links:

In this study, the obtained IC50 concentration of 51.25 μg/mL exhibits significant anticancer activity against A549 cells than other tested concentrations because of the release of zinc ions, which induce the cell apoptosis. Furthermore, studies on cell cycle validate that ZnO NPs arrest the cell cycle at the sub-G1 phase of A549 cells. These results suggest the utility of phytogenic ZnO NPs for various biomedical applications.Graphical abstract
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
The ability of human neutrophils to clear newly attached Staphylococcus aureus bacteria from a serum-coated glass surface was examined in vitro using time-lapse confocal scanning laser microscopy. Quantitative image analysis was used to measure the temporal change in bacterial biomass, neutrophil motility, and fraction of the surface area policed by neutrophils. In control experiments in which the surface was inoculated with bacteria but no neutrophils were added, prolific bacterial growth was observed. Neutrophils were able to control bacterial growth but only consistently when the neutrophil/bacterium number ratio exceed...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Bacterial Infections Source Type: research
Publication date: Available online 16 November 2019Source: Carbohydrate PolymersAuthor(s): Hossein Rahmani, Seyed Heydar Mahmoudi Najafi, Alireza Ashori, Marzieh Arab Fashapoyeh, Farzaneh Aziz Mohseni, Sara TorkamanAbstractIn this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided. The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross linker and the formation of urea linkage on mechanical properties such as tensile strength and elongation and dynamic-mechanical properties such as storage mod...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Publication date: Available online 5 November 2019Source: Inorganica Chimica ActaAuthor(s): Mousumi Nayak, Ashish Kumar Singh, Pradyot Prakash, Rajni Kant, Subrato BhattacharyaAbstractThiosalicylato complexes of Zn(II) and Hg(II), [Zn(SC6H4CO2)(TMEDA)]2 (1) and [TMEDA-H2][Hg(SC6H4CO2)2] (2) where (TMEDA = N,N,N',N'-tetramethylethylenediamine) have been synthesized and characterized by spectral and single crystal X-ray analyses. Thiosalicylate opted different binding modes; in 1 all three binding sites are used whereas in 2 bonding of the ligands are mainly through S atoms, however, Hg-O distances indicate the existence...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
Conclusion: According to the results of the present study, Dracocephalum kotschyi can be a suitable compound for the synthesis of nanostructures due to its indigenous cultivation and great medicinal properties.
Source: Current Chemical Biology - Category: Biochemistry Source Type: research
Publication date: Available online 12 November 2019Source: Materials LettersAuthor(s): Li-Jun He, Jian-Chao Hao, Lin Dai, Rong-Chang Zeng, Shuo-Qi LiAbstractTo reduce the bacteria-associated infection for the Ti implant, an antibacterial coating composed of gentamicin (GS) and polyacrylic acid (PAA) was constructed using layer-by-layer (LbL) assembly technique. The surface morphology and chemical composition of the obtained multilayers were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The plate ...
Source: Materials Letters - Category: Materials Science Source Type: research
Molecules, Vol. 24, Pages 4104: The Synergy of Ciprofloxacin and Carvedilol against Staphylococcus aureus–Prospects of a New Treatment Strategy? Molecules doi: 10.3390/molecules24224104 Authors: Katarzyna Zawadzka Marta Nowak Ireneusz Piwoński Katarzyna Lisowska Staphylococcus aureus infections are common and difficult to treat. The increasing number of drug-resistant staphylococcal infections has created the need to develop new strategies for the treatment of these infections. The synergistic antimicrobial activity of different pharmaceuticals seems to be an interesting alternative. The aim of this st...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Possible mechanisms of the antimicrobial effects of polypeptide‑enriched Gastrodia elata Blume extracts. Mol Med Rep. 2019 Sep 25;: Authors: Kong F, Cai X, Zhai S, Wang R, Zheng X, Ma Y, Bi H, Wang D Abstract The present study aimed to evaluate the antimicrobial activity and the possible mechanisms of activity of polypeptide‑enriched Gastrodia elata extracts (GEP) against the gram‑negative bacteria Escherichia coli and Pseudomonas aeruginosa, the gram‑positive bacterium Staphylococcus aureus and the fungus Candida albicans. The antimicrobial activity of GEP was first confirmed by determining th...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Publication date: Available online 6 November 2019Source: Microbial PathogenesisAuthor(s): Marcos Guilherme da Cunha, Janaina de Cássia Orlandi Sardi, Irlan Almeida Freires, Marcelo Franchin, Pedro Luiz RosalenAbstractThe discovery of new drug candidates, especially from natural products, remains a promising approach to overcome the alarmingly high microbial resistance rates. A major 4-phenyl coumarin named cinnamoyloxy-mammeisin (CNM) isolated from stingless bee geopropolis showed interesting biological properties; however, its antimicrobial activity against Staphylococcus aureus has never been investigated. In ord...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Abstract The present study focused on production, optimization and characterization of exopolysaccharide (EPS) from Weissella confusa MD1. The purified EPS MD1 was also evaluated for in vitro biological activities. The maximum yield of EPS (10.07 ± 0.32 g/L) was obtained with optimized culture conditions of 35 ˚C at pH 6.5 for 36 h with 4% (w/v) galactose and 1% (w/v) ammonium nitrate supplemented in MRS broth. The crude EPS was purified with diethylamino ethanol-Sepharose Fast Flow column and Sephadex-G 75 column. The physicochemical and functional characterization of the EPS was done by high-performance ...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
More News: Drugs & Pharmacology | PET Scan | Staphylococcus Aureus | Study