Protocol for Inferring Compound Biodegradation at Low Concentrations from Biomass Measurements

Biodegradation tests for organic compounds are usually performed at relatively high carbon concentrations (2–100 mg L−1), which can be problematic for toxic compounds. Here we describe a protocol to test compound biodegradation through the concomitant formation of bacterial biomass at relatively low carbon concentrations (0.5–5 mg C/L). The protocol is based on accurate cell counting of dilute cell suspensions by flow cytometry coupled to cell staining with fluorescent dyes.
Source: Springer protocols feed by Microbiology - Category: Microbiology Source Type: news