BDE-47 and BDE-209 inhibit proliferation of Neuro-2a cells via inducing G1-phase arrest

Publication date: Available online 21 December 2016 Source:Environmental Toxicology and Pharmacology Author(s): Hongmei Chen, Xuexi Tang, Bin Zhou, Ningning Xu, Zhongyuan Zhou, Kuan Fang, You Wang Cell proliferation is closely related to cell cycle which is strictly regulated by genes and regulatory proteins. In the present study, we comparatively analyzed the toxic effects of BDE-47 and BDE-209 on cell proliferation of Neuro-2a cells, and the possible mechanism was discussed. The results indicated that BDE-47 significantly inhibited the cell proliferation and the cell cycle were arrest at G1 phase, while BDE-209 had little effects on either cell proliferation or cell cycle. qRT-PCR and Western blot assay presented that BDE-47 up-regulated the gene expressions of p53 and p21, which down-regulated the expresseion of cyclinD1 and CDK2, and inhibited retinoblastoma protein (pRb) phosphorylation. This process could effectively arrest the cell cycle at G1 phase, which finally caused the inhibition on Neuro-2a cell proliferation. However, BDE-209 was only up-regulated the gene expressions of p53, also suggested to be involved in the inhibition on Neuro-2a cell proliferation.
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research

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