X-ray crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC

The NAD-dependent malonate-semialdehyde dehydrogenase KES23460 from Pseudomonas sp. strain AAC makes up half of a bicistronic operon responsible for β -alanine catabolism to produce acetyl-CoA. The KES23460 protein has been heterologously expressed, purified and used to generate crystals suitable for X-ray diffraction studies. The crystals belonged to space group P212121 and diffracted X-rays to beyond 3   Å resolution using the microfocus beamline of the Australian Synchrotron. The structure was solved using molecular replacement, with a monomer from PDB entry 4zz7 as the search model.

Source: Acta Crystallographica Section F - December 24, 2016 Category: Biochemistry Authors: Wilding, M. Scott, C. Peat, T.S. Newman, J. Tags: malonate-semialdehyde dehydrogenase β -alanine catabolism Pseudomonas sp. strain AAC research communications Source Type: research