Genomic sequencing gives insight into Shigella outbreaks

Amy WallaceSACRAMENTO, Dec. 23 (UPI) -- Researchers have sequenced and analyzed genomes from bacteria associated with major shigellosis outbreaks in California in 2014 and 2015.
Source: Health News - UPI.com - Category: Consumer Health News Source Type: news

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AbstractA gastroenteritis outbreak occurred in a university in May, 2017, Wuhan, China. The epidemiological survey and pathogen analysis were conducted to identify the pathogen and control this outbreak. Feces or anal swabs from individuals, water, and swabs taken from tap surfaces of the secondary water supply system (SWSS) and foods were collected for the detection of viruses and pathogenic enteric bacteria by real-time RT-PCR and culture, respectively. Nucleotide sequences were determined by RT-PCR and direct sequencing. Genotyping, phylogenetic, and recombination analyses were conducted by a web-based genotyping tool, ...
Source: Food and Environmental Virology - Category: Virology Source Type: research
S, Bidet P Abstract We developed a multiplex PCR method based on Multiple-Locus Variable-number of tandem repeats (VNTR)-Analysis (MLVA) designed for rapid typing of E. coli and Shigella isolates The method amplifying seven VNTRs doesn't require sequencing capillary nor fluorescent dyes. Amplification products are simply loaded on a standard agarose gel for electrophoresis and banding patterns are analyzed visually. We evaluated the method on 220 strains belonging to different collections: ECOR collection (n=72), O1:K1 isolates causing neonatal meningitis (n=38), extended-spectrum beta-lactamase-producing fecal i...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Abstract In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the ipaH gene in 10/12 faecal specimens, and Shigella boydii serotype 20 was isolated from 7 patients. A case control study was undertaken and analysed using multivariable logistic regression. Consumption of a coronation chicken lunch at the transit camp in Sierra Leone (SL) 24-48 h prior t...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
More than 600 people said they got sick after eating at a Chipotle outside Columbus, Ohio, health officials said Monday. And they still don’t know what has caused it. The Delaware General Health District began investigating the restaurant, located in Powell, Ohio, on July 31 after it received numerous reports from patrons who ate there between July 26 and July 30. The location was briefly closed for an investigation. On Aug. 3, health officials put out an update saying that they had received nearly 700 inquires and had interviewed over 500 people. On Monday, they provided another update on Twitter saying that officia...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized Food & Drink Ohio onetime Source Type: news
Background: Shigella infection is more frequent during times of heat or heavy rains. Shigellosis mainly affects children under the age of ten. Man is the only reservoir of Shigella. Most cases are produced by person-to-person transmission through the fecal-oral route. Epidemiologycal outbreaks may occur if food contamination with faeces of patients with shigellosis occurs.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: UMP. 660 Source Type: research
Abstract The highly virulent Escherichia coli O104:H4 isolate that caused a large outbreak in 2011 carries three plasmids. Out of these, only one, the IncI plasmid pO104_90 that encodes two extended spectrum beta-lactamases, can transfer itself by conjugation. Considering its potential contribution to the emergence and virulence of the outbreak strain, we aimed to get a closer insight into pO104_90 transfer efficiency and control. We tested the host spectrum of the plasmid and observed transmission into Enterobactericeae including clinically relevant enterobacterial pathogens like Salmonella typhimurium and Shigel...
Source: International Journal of Medical Microbiology - Category: Microbiology Authors: Tags: Int J Med Microbiol Source Type: research
Abstract Outbreaks associated with untreated recreational water can be caused by pathogens, toxins, or chemicals in fresh water (e.g., lakes, rivers) or marine water (e.g., ocean). During 2000-2014, public health officials from 35 states and Guam voluntarily reported 140 untreated recreational water-associated outbreaks to CDC. These outbreaks resulted in at least 4,958 cases of disease and two deaths. Among the 95 outbreaks with a confirmed infectious etiology, enteric pathogens caused 80 (84%); 21 (22%) were caused by norovirus, 19 (20%) by Escherichia coli, 14 (15%) by Shigella, and 12 (13%) by Cryptosporidium....
Source: MMWR Morb Mortal Wkl... - Category: Epidemiology Authors: Tags: MMWR Morb Mortal Wkly Rep Source Type: research
Just in time for swimming season, the Centers for Disease Control and Prevention (CDC) has issued a new report on disease outbreaks associated with swimming in rivers, lakes and oceans. Between 2000 and 2014, public health officials in 35 states, plus Guam, reported a total of 140 disease outbreaks associated with swimming in untreated recreational waters — mainly at beaches and bodies of water in public parks, the report says. These outbreaks led to 4,958 illnesses and two deaths. The vast majority of outbreaks with a confirmed cause were linked to gastrointestinal pathogens such as norovirus, Shigella and E. coli. ...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized healthytime onetime public health Source Type: news
A. Behar et al.
Source: Emerging Infectious Diseases Journal - Category: Infectious Diseases Source Type: research
Members of the genus Shigella are intestinal pathogens and a major cause of seasonal outbreaks of bacterial diarrhea worldwide. Although humans are the conventional hosts of Shigella species, expansion of the Shi...
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Research article Source Type: research
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