Synchronized Fast SPE and UFLC Methods for the Analyses of Eight Antidiabetic Drugs in Human Plasma.

Synchronized Fast SPE and UFLC Methods for the Analyses of Eight Antidiabetic Drugs in Human Plasma. Comb Chem High Throughput Screen. 2016 Dec 20; Authors: Ali I, Dutta K, Jain AK Abstract The number of the diabetic patients is increasing rapidly globally. The treatment of these patients is a complex phenomenon due to the use of the different drugs. The present article reports a synchronized fast SPE-UFLC separation of eight antidiabetic drugs in human plasma. The separated drugs include metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and repaglinide. The column used was Sunshell C18 (150 x 4.6 mm, 2.6 µm) with mobile phase of acetate buffer (0.05% TEA in 0.05 M ammonium acetate of pH 7.0 with H 3PO4)-acetonitrile (60:40, v/v). The flow rate was 1.0 mL/min with detection wavelength 210 nm and column temperature 45±1ºC. The values for the retention, separation and resolution factors were ranged from 0.072 to 9.14, 1.44 to 4.21 and 2.15 to 18.66, correspondingly. The percent recoveries for these drugs in the standard laboratory samples prepared in water were ranged from 77 to 88%. These values in plasma samples were ranged from 10 to 22%. The validated method was successfully applied to analyze these drugs in human plasma for the clinical monitoring of these drugs. PMID: 28000562 [PubMed - as supplied by publisher]
Source: Combinatorial Chemistry and High Throughput Screening - Category: Chemistry Authors: Tags: Comb Chem High Throughput Screen Source Type: research