Cell cycle arrest and apoptosis induced by enteroaggregative Escherichia coli in cultured human intestinal epithelial cells.

Cell cycle arrest and apoptosis induced by enteroaggregative Escherichia coli in cultured human intestinal epithelial cells. J Med Microbiol. 2016 Dec 09;: Authors: Priya A, Kaur K, Bhattacharyya S, Chakraborti A, Ghosh S Abstract Enteroaggregative Escherichia coli (EAEC) is an emerging food borne enteric pathogen causing diarrheal diseases in multiple epidemiological and clinical settings. However, understanding of the pathogenesis of EAEC is still suboptimal. Studies have indicated that enteric bacteria induced cell cycle arrest and apoptosis in host cells might have a vital role in the pathogenesis caused by these organisms. In the present study, we have found that EAEC could induce apoptosis in human small intestinal as well as colonic epithelial cell lines, which was assessed by the expression of phosphatidylserine on host cell surface, internucleosomal cleavage of host cell DNA and microscopic analysis of the characteristic apoptotic features of these cells. EAEC was also found to arrest cells at S phase and G2-M phase of the cell cycle. Further, we have observed that the plasmid of this organism had maximum contribution to these processes. To our knowledge, this is the first report regarding EAEC induced cell cycle modulation and apoptosis in human intestinal epithelial cell lines. The outcome of this study has undoubtedly led to a better understanding of the basic mechanism of pathogenesis caused by EAEC. PMID: 279597...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research