Effects of Mn-doping on the structure and biological properties of β-tricalcium phosphate.

Effects of Mn-doping on the structure and biological properties of β-tricalcium phosphate. J Inorg Biochem. 2014 Apr 3;136C:57-66 Authors: Torres PM, Vieira SI, Cerqueira AR, Pina S, da Cruz Silva OA, Abrantes JC, Ferreira JM Abstract Doping calcium phosphates with trace elements that exist in bone tissues is beneficial in terms of cell-material interactions and in vivo performance of the bone grafts made thereof. Manganese (Mn) is an essential element for normal growth and metabolism of bone tissues, but studies reporting the effects of Mn-doping calcium phosphates are scarce. The present study investigated the influence of Mn-doping on the structure, morphology and biological properties of β-tricalcium phosphate [β-Ca3(PO4)2] (β-TCP). Mn-doped (MnTCP) powders, with Mn contents varying from 0 to 10mol%, were obtained through an aqueous precipitation method followed by heat treatment at 800°C. The successful incorporation of Mn into β-TCP structure was proved through quantitative X-ray diffraction (XRD) phase analysis coupled with structural Rietveld refinement. Increasing Mn concentrations led to decreasing trends of a- and c-axis lattice parameters, and Mn-doping also significantly affected the morphology of β-TCP powders. In vitro proliferation and differentiation assays of MC3T3-E1 osteoblastic-like cells, grown in the presence of the powders, revealed that the biological benefits of Mn-doped β-TCP are limited to lower Mn...
Source: Journal of Inorganic Biochemistry - Category: Biochemistry Authors: Tags: J Inorg Biochem Source Type: research