Mycobacterium malmesburyense sp. nov: A novel non-tuberculous Mycobacterium species revealed by multiple gene sequence characterization.

In this study we characterized 10 NTM isolates originating from water, soil, nasal swabs of cattle and African buffalo as well as bovine tissue samples. These isolates were previously identified during an NTM survey and were all found, using 16S rDNA sequence analysis to be closely-related to Mycobacterium moriokaense. A polyphasic approach that includes phenotypic characterization, antibiotic susceptibility profiling, mycolic acid profiling and phylogenetic analysis of four gene loci, viz 16S rDNA, hsp65, sodA, and rpoB was employed to characterize these isolates. Sequence data analysis of the four gene loci revealed that these isolates belong to a unique Mycobacterium species. This evidence was further supported by several differences in phenotypic characteristics between the isolates and the closely related species. We proposed the name, Mycobacterium malmesburyense sp. nov. for this new species. The type strain is WCM 7299T (ATCC® BAA-2759TM =CIP 110822T). The Genbank accession numbers for the partial gene sequences [16S rDNA , hsp65, rpoB and sodA] for the type strain are as follows: 16S rRNA= KJ 873241; hsp65=KJ 873243; rpoB= KJ 873245; sodA= KJ 873247. PMID: 27926825 [PubMed - as supplied by publisher]
Source: International Journal of Systematic and Evolutionary Microbiology - Category: Microbiology Authors: Tags: Int J Syst Evol Microbiol Source Type: research