Development of an RT-qPCR assay for the specific detection of a distinct genetic lineage of the infectious bursal disease virus.

Development of an RT-qPCR assay for the specific detection of a distinct genetic lineage of the infectious bursal disease virus. Avian Pathol. 2016 Dec 07;:1-7 Authors: Tomás G, Hernández M, Marandino A, Techera C, Grecco S, Hernández D, Banda A, Panzera Y, Pérez R Abstract The infectious bursal disease virus (IBDV) is a major health threat to the world's poultry industry despite intensive controls including proper biosafety practices and vaccination. IBDV (Avibirnavirus, Birnaviridae) is a non-enveloped virus with a bisegmented double-stranded RNA genome. The virus is traditionally classified into classic, variant and very virulent strains, each with different epidemiological relevance and clinical implications. Recently, a novel worldwide spread genetic lineage was described and denoted as distinct (d) IBDV. Here, we report the development and validation of a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay for the specific detection of dIBDVs in the global poultry industry. The assay employs a TaqMan-MGB probe that hybridizes with a unique molecular signature of dIBDV. The assay successfully detected all the assessed strains belonging to the dIBDV genetic lineage, showing high specificity and absence of cross-reactivity with non-dIBDVs, IBDV-negative samples and other common avian viruses. Using serial dilutions of in vitro-transcribed RNA we obtained acceptable PCR efficiencies and determination co...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research