Infectious bronchitis vaccine virus detection and part-S1 genetic variation following single or dual inoculation in broiler chicks.

Infectious bronchitis vaccine virus detection and part-S1 genetic variation following single or dual inoculation in broiler chicks. Avian Pathol. 2016 Dec 05;:1-26 Authors: Ball C, Awad F, Hutton S, Forrester A, Baylis M, Ganapathy K Abstract An investigation was undertaken of the extent of genetic variation occurring within infectious bronchitis virus (IBV) vaccine strains following vaccination of day-old broiler chicks. Chicks were divided into seven groups, with two groups receiving single Massachusetts (Mass) vaccinations and the other four were inoculated with combinations of different IBV serotypes; Mass, 793B, D274, and Arkansas (Ark). The remaining group was maintained as an unvaccinated control. Following vaccination, swabs and tissues collected at intervals were pooled and RNA was extracted for detection of IBV by reverse transcription polymerase chain reaction (RT PCR). Positive amplicons were sequenced for the part-S1 gene and compared to the original vaccine strain sequences. Single nucleotide polymorphisms (SNPs), amino acid variations and hydrophobicity changes were identified and recorded for each sampling point. A total of 106 SNPs were detected within 28 isolates. The average SNP counts of swab isolates were greater than those found in tissue samples. This translated into 64 amino acid changes, however only six resulted in a change to the hydrophobicity properties. All hydrophobic alterations occurred within swab is...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research