Antimicrobial resistance profiling and molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) isolated from a Malaysian Teaching Hospital.

Antimicrobial resistance profiling and molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) isolated from a Malaysian Teaching Hospital. J Med Microbiol. 2016 Nov 07;: Authors: Noordin A, Sapri HF, Mohamad Sani NA, Leong SK, Tan XE, Tan TL, Mohamad Zin N, Neoh HM, Hussin S Abstract The annual prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Malaysia has been estimated to be at 30-40% of all S. aureus infections. Nevertheless, data on the antimicrobial resistance and genetic diversity of Malaysian MRSAs remain few. In 2009, we collected 318 MRSA strains from various wards of our teaching hospital located in Kuala Lumpur, the capital city of Malaysia, and performed antimicrobial susceptibility testing on the strains. The strains were then molecularly characterized via SCCmec and virulence gene (cna, sea, seb, sec, sed, see, seg, seh, sei, eta, etb, PVL and TSST-1) typing; a subset of 49 strains isolated from the Intensive Care Unit (ICU) were also typed using Pulsed-Field Gel Electrophoresis (PFGE). Most strains were found to be resistant to ciprofloxacin (92.5%), erythromycin (93.4%) and gentamicin (86.8%). Majority (72.0%) of our strains were found to harbour SCCmec type III-SCCmercury with the presence of ccrC, and carried the sea+cna gene combination (49.3%), with cna as the most prevalent virulence gene (94.0%) detected. We identified 4 PFGE clusters, with pulsotype C (n=19) as the dominant pul...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research