Clonal spread and accumulation of Beta-lactams resistance determinants in Enterobacter aerogenes and Enterobacter cloacae complex isolates from infection and colonization in patients at a public hospital in Recife, Pernambuco, Brazil.

This study has characterized phenotypically and genotypically 51 isolates of E. aerogenes and E. cloacae complex as originated from infection or colonization in patients admitted to public hospital in Recife, Pernambuco, Brazil, by antimicrobial susceptibility profile, analysis of β-lactamase genes (blaTEM, blaSHV, blaCTX-M, blaKPC, blaVIM, blaIMP and blaSPM), PCR and DNA sequencing, plasmid profile and ERIC-PCR. In both species, the genes blaTEM, blaCTX-M and blaKPC were detected. The DNA sequencing confirmed the variants blaTEM-1, blaCTX-M-15 and blaKPC-2 in isolates. It was observed more than one genes conferring resistance in the isolates, including the detection of the three previously cited genes in strains isolated from infection sites. The detection of blaCTX-M was more frequent in isolates from infection sites than from colonization. The blaKPC predominated in E. cloacae complex isolates obtained from infections, however in E. aerogenes isolates it predominated in samples obtained from colonization. Clonal relationship among all of E. aerogenes isolates was detected by ERIC-PCR. The majority of E. cloacae complex isolates presented the same ERIC-PCR pattern. Despite the clonal relation that the isolates presented by ERIC-PCR, it was observed different plasmid and resistance profiles, and several resistance genes. The clonal dissemination and the accumulation of beta-lactams resistance determinants presented by the isolates demonstrated the ability of E. aerogenes an...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research