A high-order representation and classification method for transcription factor binding sites recognition in Escherichia coli

Conclusion The representation method for TFs is an important step in TFBSs recognition. We illustrate the benefits of this representation on real data application via a series of experiments. This method can gain further insights into the mechanism of TF binding and be of great use for metabolic engineering applications.
Source: Artificial Intelligence in Medicine - Category: Bioinformatics Source Type: research

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Authors: DENTAL SUPPLEMENT, Oberti L, Gabrione F, Greco G, Severino M Abstract Osseointegrated dental implants showed elevated success rates on the long-term treatment in the last ten years. However, the risk of peri-implantitis and implant failure is the main complication of implantology. The presence of a micro gap at the implant-abutment connection (IAC) allows microorganisms to penetrate and colonize the inner part of the implant leading to biofilm accumulation and consequently to peri-implantitis development. Some chemical devices (CD) has been studied to reduce bacterial penetration at IAC level but no one ha...
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research
Publication date: January 2020Source: LWT, Volume 117Author(s): Haiying Cui, Chenghui Zhang, Changzhu Li, Lin LinAbstractEscherichia coli O157:H7 (E. coli O157:H7) easily forms biofilms on pores and veins of plant, which poses a serious hazard to safety of ready-to-eat vegetables. Therefore, this study was designed to evaluate inhibition mechanism of clove oil on E. coli O157:H7 biofilm and apply it to vegetable for reduce risk of contamination. The results of crystal violet staining showed that clove oil could effectively remove biofilm. After treatment of biofilm with clove oil (2 MBIC), the results of resazurin method s...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: January 2020Source: LWT, Volume 117Author(s): A. García, L.M. Pérez, G.N. Piccirilli, R.A. VerdiniAbstractSoy sauce is a traditional seasoning for foods available at the global market that is consumed all over the world. This sauce has a unique flavor and also antimicrobial and antioxidant properties. There is a wide range of soy sauces and their differences are mainly based on the manufacturing methods. Variation in chemical composition is mainly due to differences in fermentation process and the addition of supplementary ingredients like flavor enhancers, among others. The present work, fi...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: January 2020Source: LWT, Volume 117Author(s): H. Staroszczyk, B. Kusznierewicz, E. Malinowska-PaƄczyk, I. Sinkiewicz, K. Gottfried, I. KoƂodziejskaAbstractThe aim of the work was to study the feasibility of using aqueous extracts from rowanberry, blue-berried honeysuckle, and chokeberry pomace for the formulation of fish gelatin films with antioxidant and antimicrobial activity as well as improved mechanical and water barrier properties. The predominant phenolic components in rowanberry and chokeberry extracts were hydroxycinnamates, and in blue-berried honeysuckle extract anthocyanins. Although the gel...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 20 September 2019Source: Journal of BiotechnologyAuthor(s): Jonathan Guyang Ling, Muhamad Hawari Mansor, Abdul Munir Abdul Murad, Rozida Mohd. Khalid, Doris Huai Xia Quay, Margit Winkler, Farah Diba Abu BakarAbstractCarboxylic acid reductases (CARs) are attracting burgeoning attention as biocatalysts for organic synthesis of aldehydes and their follow-up products from economic carboxylic acid precursors. The CAR enzyme class as a whole, however, is still poorly understood. To date, relatively few CAR sequences have been reported, especially from fungal sources. Here, we sought to increase...
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research
Publication date: Available online 20 September 2019Source: Journal of BiotechnologyAuthor(s): Gabriela Pannunzio Carmignotto, Adriano Rodrigues AzzoniAbstractThe CRISPR-Cas9 system is a new tool that has been extensively used for genome editing. The system is composed of a Cas9 endonuclease, which has the function of cleaving DNA at a specific site, and a guide RNA (gRNA), which contains the sequence of the cleavage site that is the target of editing. Despite the great interest that has been generated because of the utility of Cas 9 as a molecular tool and a potential therapeutic protein, the production of the 158 ...
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research
In this study, the gene encoding CUL-I was cloned, and the recombinant enzyme was heterologously expressed in Escherichia coli. The predicted CUL-I protein is composed of 426 amino acid residues and includes a putative 21-amino acid signal peptide. The recombinant CUL-I specifically depolymerized β-1,4-glycoside linkages of cellouronate, and its mode of action was endo-type, like the native CUL-I. Sequence analysis showed CUL-I has no similarity to previously known polysaccharide lyases (PLs), indicating that CUL-I should be classified into a novel PL family.
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Conclusions: Low-dose of domperidone with ORT did not significantly differ from placebo in reducing vomiting and nausea episodes in pediatric patients with acute gastroenteritis (AG), and the safety profile was similar between both groups.
Source: Journal of Pediatric Gastroenterology and Nutrition - Category: Gastroenterology Tags: Original Articles: Gastroenterology Source Type: research
Conclusions: These results demonstrate that genotype G1P[8] is the most prevalent strain. VP7 and/or VP8* gene segments arising from RV1 vaccine strain were documented in these children, suggesting shedding or herd vaccination. Moreover, our study indicates full vaccination is important for protection against RVA infections.
Source: Journal of Pediatric Gastroenterology and Nutrition - Category: Gastroenterology Tags: Original Articles: Gastroenterology Source Type: research
Authors: Fukushima K, Yanagisawa N, Sekiya N, Izumiya H Abstract Non-typhoidal Salmonella (NTS) infection is a major pathogen causing gastroenteritis among immunocompetent adults. NTS infection is mainly transmitted by contaminated food and water, but some cases are transmitted by animal contact. Salmonella enterica subsp. enterica serovar Poona (S. Poona) is an NTS usually transmitted by reptiles, and cases including outbreaks of gastroenteritis have been reported previously. However, invasive infections due to this organism among immunocompetent adults are rare. We herein report a case of a 39-year-old man who wa...
Source: Internal Medicine - Category: Internal Medicine Tags: Intern Med Source Type: research
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