Simultaneous Determination of Glycyrrhizin and Liquiritin in Licorice Roots and Kampo medicines by Combination Enzyme-linked Immunosorbent Assay using anti-Glycyrrhizin and anti-Liquiritin Monoclonal Antibodies.

In this study, a combination enzyme-linked immunosorbent assay (ELISA) using both anti-glycyrrhizin and anti-liquiritin mAbs (anti-GL/Liq mixture mAbs) was developed for quality control of licorice and its products. The combination ELISA demonstrated high sensitivity, reproducibility, and specificity for the total content of GL and Liq by a single assay. The developed ELISA was effective and useful as the first screening method in the selection of high-quality licorice from the Glycyrrhiza species and in confirming the quality of licorice-containing Kampo medicines.
PMID: 27892811 [PubMed - as supplied by publisher]

Source: Journal of Immunoassay and Immunochemistry - November 30, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research