Effects of RNAi-mediated TUSC3 silencing on radiation-induced autophagy and radiation sensitivity of human lung adenocarcinoma cell line A549 under hypoxic condition

This study examined the effects of RNAi-mediated TUSC3 silencing on radiation-induced autophagy and radiation sensitivity of human lung adenocarcinoma cell line A549 under hypoxic condition. Different CoCl2 concentrations were used to treat A549 cells and establish a CoCl2-induced hypoxic model of A549 cells. MTT and clone formation assays were used to determine the effects of different concentrations of CoCl2 on the growth and proliferation of A549 cells treated by different doses of X-ray irradiation. The siRNA-expressing vector was transfected by liposomes and for silencing ofTUSC3. Flow cytometry was used to measure cell cycle changes and apoptosis rate. Real-time quantitative polymerase chain reaction (qRT-PCR) assay was performed to detect the expression of TUSC3 mRNA. Western blotting was applied to detect the changes of TUSC3, LC3, and p62 proteins under different CoCl2 concentrations and after siRNA silencing ofTUSC3. The TUSC3 levels in A549 cells increased under hypoxic conditions in a dose-dependent manner (P <  0.05). Hypoxia inhibited the growth and proliferation of A549 cells and promoted apoptosis (P <  0.05). With an increasing dose of X-ray irradiation, A549 cells showed significantly increased growth and proliferation and decreased apoptosis (P <  0.05). After siRNA-TUSC3 was transfected by liposome, the TUSC3 level was substantially inhibited (P <  0.05). SilencingTUSC3 inhibited A549 cell growth and proliferation after radiotherapy und...
Source: Tumor Biology - Category: Cancer & Oncology Source Type: research