The Expression of IGFBP6 after Spinal Cord Injury: Implications for Neuronal Apoptosis.

In this study, we have performed an acute SCI model in adult rats and investigated the dynamic changes of IGFBP6 expression in the spinal cord. Our results showed that IGFBP6 was upregulated significantly after SCI, which was paralleled with the levels of apoptotic proteins p53 and active caspase-3. Immunofluorescent labeling showed that IGFBP6 was co-localizated with active caspase-3 and p53 in neurons. To further investigate the function of IGFBP6, an apoptosis model was established in primary neuronal cells. When IGFBP6 was knocked down by specific short interfering RNA (siRNA), the protein levels of active caspase-3 and Bax as well as the number of apoptotic primary neurons were significantly decreased in our study. Taken together, our findings suggest that the change of IGFBP6 protein expression plays a key role in neuronal apoptosis after SCI. PMID: 27888466 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/27888466?dopt=Abstract

Source: Neurochemical Research - November 25, 2016 Category: Neuroscience Authors: Wang S, Liu Y, Wu C, Zhao W, Zhang J, Bao G, Xu G, Sun Y, Chen J, Cui Z Tags: Neurochem Res Source Type: research