Application of DoE approach in the development of mini-capsules, based on biopolymers and manuka honey polar fraction, as powder formulation for the treatment of skin ulcers

Publication date: 10 January 2017 Source:International Journal of Pharmaceutics, Volume 516, Issues 1–2 Author(s): Marika Tenci, Silvia Rossi, Maria Cristina Bonferoni, Giuseppina Sandri, Irene Mentori, Cinzia Boselli, Antonia Icaro Cornaglia, Maria Daglia, Anna Marchese, Carla Caramella, Franca Ferrari The aim of the present work was the development of a powder formulation for the delivery of manuka honey (MH) bioactive components in the treatment of chronic skin ulcers. In particular pectin (PEC)/chitosan glutamate (CS)/hyaluronic acid (HA) mini-capsules were obtained by inverse ionotropic gelation in presence of calcium chloride and subsequently freeze-dried. Optimization of unloaded (blank) formulation was performed using DoE approach. In a screening phase, the following three factors were investigated at two levels: CS (0.5–1% w/w), PEC (0.5–1% w/w) and HA (0.3–0.5% w/w) concentrations. For the optimization phase a “central composite design” was used. The response variables considered were: particle size, buffer (PBS) absorption and mechanical resistance. In a previously work two different MH fractions were investigated, in particular MH fraction 1 (Fr1), rich in polar substances (sugars, methylglyoxal (MGO), dicarbonyl compounds, …), was able to enhance human fibroblasts in vitro proliferation. In the present work, the loading of MH Fr1 into mini-capsules of optimized composition determined a significant increase in cell proliferation in compa...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research