Harnessing the power of predatory bacteria as a 'living antibiotic'

(Wellcome Trust) A naturally occurring predatory bacterium is able to work with the immune system to clear multi-drug resistant Shigella infections in zebrafish, according to a study published today in Current Biology.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news

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The enteric bacterium and intracellular human pathogen Shigella causes hundreds of millions of cases of the diarrheal disease shigellosis per year worldwide. Shigella is acquired by ingestion of contaminated food or water; upon reaching the colon, the bacteria invade colonic epithelial cells, replicate intracellularly, spread to adjacent cells, and provoke an intense inflammatory response. There is no animal model that faithfully recapitulates human disease; thus, cultured cells have been used to model Shigella pathogenesis. However, the use of transformed cells in culture does not provide the same environment to the bacte...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research
In this study, we show that Shigella flexneri is capable of infecting and replicating intracellularly in human enteroids derived from different segments of the intestine. Apical invasion by S. flexneri is very limited but increases ~10-fold when enteroids are differentiated to include M cells. Invasion via the basolateral surface was at least 2-log10 units more efficient than apical infection. Increased secretion of interleukin-8 and higher expression levels of the mucin glycoprotein Muc2 were observed in the enteroids following S. flexneri infection. The human enteroid model promises to bridge some of the gaps between tra...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Bacterial Infections Source Type: research
Publication date: Available online 21 March 2019Source: MethodsAuthor(s): Navoun Silué, Endrei Marcantonio, F-X Campbell-ValoisAbstractShigella spp. are enterobacteria that invade human colonic mucosal cells using their Type Three Secretion Apparatus (T3SA). Shigella spp. possess a large plasmid that encodes most of its virulence factors and has been the focus of seminal work that defined the T3SA regulon. Thus, a global assessment of the transcriptional response regulated by the T3SA has been lacking. Herein we used RNA-Seq to identify genes that are differentially expressed when the T3SA is active (on-state) versu...
Source: Methods - Category: Molecular Biology Source Type: research
We report 2 cases of HUS, respectively, caused by salmonella and Campylobacter jejuni infections. None of these bacteria produce shigatoxins, and the underlying mechanism of HUS development remains unknown. In streptococcus pneumoniae–associated HUS, bacterial neuraminidase cleaves neuraminic acid and causes exposure of Thomsen-Friedenreich cryptantigen on the cell surface of, for example, erythrocytes, which induces an inflammatory response caused by binding of preformed IgM. Both campylobacter and salmonella bacteria also produce neuraminidase, and HUS development could be explained by a similar mechanism.
Source: Journal of Pediatric Hematology Oncology - Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research
Publication date: Available online 20 March 2019Source: LWTAuthor(s): Leonardo Luiz de Freitas, Cláudia Vieira Prudêncio, Wilmer Edgard Luera Peña, Maria Cristina Dantas VanettiAbstractThe Response Surface Methodology (RSM) was used to establish a predictive model of inactivation of S. flexneri using ultrasound time of 5–20 min, pH of 4.0–7.0 and nisin concentration of 29.1–291.1 μM as independent variables. In addition, a survival of S. flexneri at 7 °C for 120 h after treatment with ultrasound and nisin was evaluated as well as the ATP leakage. The response surface mod...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
ConclusionCooked mixtures of meat and RS are promising for developing novel functional meat products for nutritional health interventions.
Source: Molecular Nutrition and Food Research - Category: Food Science Authors: Tags: Research Article Source Type: research
ConclusionGut microbiota composition was explored to be related to the occurrence of biopsy-proven DN from DM. DM could be distinguished from HC by detectingg_Prevotella_9 level in feces, while DN was different from DM by the variables ofg_Escherichia-Shigella andg_Prevotella_9, which potentially contributed to the physiopathological diagnosis of DN from DM.
Source: Acta Diabetologica - Category: Endocrinology Source Type: research
Publication date: Available online 12 March 2019Source: Journal of Global Antimicrobial ResistanceAuthor(s): Francesco Luzzaro, Mathieu Clément, Luigi Principe, Valentina Viaggi, Odette J. Bernasconi, Andrea Endimiani
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Microbial Drug Resistance,Volume 25, Issue 2, Page 212-218, March 2019.
Source: Microbial Drug Resistance - Category: Microbiology Authors: Source Type: research
Abstract Septins are cytoskeletal proteins widely recognized for their role in eukaryotic cell division. Septins also assemble into cage-like structures that entrap cytosolic Shigella flexneri targeted to macroautophagy/autophagy. Although the Shigella septin cage was discovered ~10 y ago, how septins recognize Shigella was poorly understood. We found that septins are recruited to regions of micrometer-scale curvature presented by dividing bacterial cells, and cardiolipin (a curvature-specific phospholipid) promotes septin recruitment to these regions. Chemical manipulation of bacteria revealed that following...
Source: Autophagy - Category: Cytology Authors: Tags: Autophagy Source Type: research
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