Laboratory Investigation of Salmonella enterica serovar Poona Outbreak in California: Comparison of Pulsed-Field Gel Electrophoresis (PFGE) and Whole Genome Sequencing (WGS) Results

Conclusions Real-time PFGE and WGS genotyping results from this Salmonella Poona outbreak provided concordant assignment of isolates to the outbreak cluster by both methods. However, the WGS allowed for a more unequivocal determination of the genetic relatedness of the isolates than PFGE. WGS provided additional information regarding MLST type, pathogenicity genes, and bacteriophage content of the isolates. WGS data obtained independently at two laboratories showed complete agreement. Data Availability Statement The raw reads for sequenced here samples were submitted to NCBI SRA archive under accession numbers SRR2566826- SRR2566828, SRR2566830, SRR3086799- SRR3086806, SRR3086823, SRR3086828, SRR3086831, SRR3086834, SRR3095255 (see Table 1 for details). Competing Interest Statement The authors have declared that no competing interests exist. Corresponding Authors Vishnu Chaturvedi: Vishnu.Chaturvedi@cdph.ca.gov Varvara K. Kozyreva: Varvara.Kozyreva@cdph.ca.gov
Source: PLOS Currents Outbreaks - Category: Epidemiology Authors: Source Type: research