Cyclic di-AMP Released from Staphylococcus aureus Biofilm Induces a Macrophage Type I Interferon Response Host Response and Inflammation

This study examined the possibility that c-di-AMP released during S. aureus biofilm growth polarizes macrophages toward an anti-inflammatory phenotype via type I interferon signaling. DacA, the enzyme responsible for c-di-AMP synthesis in S. aureus, was highly expressed during biofilm growth, and 30 to 50% of total c-di-AMP produced from S. aureus biofilm was released extracellularly due to autolytic activity. S. aureus biofilm c-di-AMP release induced macrophage type I IFN expression via a STING-dependent pathway and promoted S. aureus intracellular survival in macrophages. These findings identify c-di-AMP as another mechanism for how S. aureus biofilms promote macrophage anti-inflammatory activity, which likely contributes to biofilm persistence.
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Host Response and Inflammation Source Type: research

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Publication date: Available online 20 January 2020Source: LWTAuthor(s): Haiying Cui, Chenghui Zhang, Changzhu Li, Lin LinAbstractIn recent years, studies have found that in addition to nosocomial infections, methicillin-resistant Staphylococcus aureus (MRSA) can even cause community-associated infections, and MRSA spreads through food and animal that produce products in the community. In addition, MRSA can colonize the surface of food and food container and form biofilms by secreting extracellular polymers, leading to cross-infection. Cardamom essential oil has been found to be highly active against MRSA. Therefore, this s...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
In this study, three 3D-supra-molecular coordination compounds namely, [Ag2L(NO3)2]n (1), {[Ag2L][PF6]2}n (2) and [AgLBr]n (3) (L =1,1’-(1,5-pentadienyl)bis-(1,3-dihydro-3-methyl-1H-imidazole-2-thione)) have been synthesized and fully characterized via a single crystal X-ray diffraction, powder X-ray diffraction (PXRD), elemental analysis (CHN), FT-IR spectra and thermo gravimetric analysis (TGA). Structural analysis revealed that the counter ions have a notable impact in directing the conformation and coordination mode of ligand, but they have no effect on the structural dimension of the polymers. Furthermore, by in...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
In this study, three tribenzoporphyrazines with dendrimeric peripheral substituents were subjected to in vitro antimicrobial photocytotoxicity study. One new magnesium(II) tribenzoporphyrazine with peripheral 3,5-bis(3,5-dimethoxybenzyloxy)benzylsulfanyl substituents was synthesized and subjected to physicochemical characterization using NMR, UV–Vis, and mass spectrometry techniques. In photochemical studies this molecule revealed moderate singlet oxygen generation ability (ΦΔDMF = 0.12, ΦΔDMSO = 0.13). The other two magnesium(II) tribenzoporphyrazines applied in the biological study were ...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Publication date: Available online 20 January 2020Source: Microbial PathogenesisAuthor(s): Sayedeh Saleheh Vaezi, Elahe Poorazizi, Arezoo Tahmourespour, Farham AminshareiAbstractBiofilms are organized communities, adherent to the surface and resistant to adverse environmental and antimicrobial agents. So, its control is very important. Staphylococcus aureus is an opportunistic pathogen with the biofilm-forming ability that causes numerous problems in the medicine and food industry. Therefore, this study aimed to investigate the effect of pH, ethanol and NaCl concentrations after 24 and 48 h incubation times at 37 °...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
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Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Archivos de la Sociedad Espanola de Oftalmologia - Category: Opthalmology Tags: Arch Soc Esp Oftalmol Source Type: research
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Source: M2E Too! Mellick's Multimedia EduBlog - Category: Emergency Medicine Tags: Blog Posts Source Type: blogs
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Source: Surgical Neurology International - Category: Neurosurgery Tags: Surg Neurol Int Source Type: research
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Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
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