The Role of the < b > < i > ydiB < /i > < /b > Gene, Which Encodes Quinate/Shikimate Dehydrogenase, in the Production of Quinic, Dehydroshikimic and Shikimic Acids in a PTS < sup > - < /sup > Strain of < b > < i > Escherichia coli < /i > < /b >

We report the effect of the inactivation or the overexpression ofydiB inE. coli strain PB12.SA22 on SA, QA, and DHS production in batch fermentor cultures. The results showed that the inactivation ofydiBresulted in a 75% decrease in the molar yield of QA and a 6.17% reduction in the yield of QA (mol/mol) relative to SA with respect to the parental strain. The overexpression ofydiB caused a 500% increase in the molar yield of QA and resulted in a 152% increase in QA (mol/mol) relative to SA, with a sharp decrease in SA production. Production of SA, QA, and DHS in parental and derivativeydiBstrains suggests that the synthesis of QA results from the reduction of 3-dehydroquinate by YdiB before its conversion to DHS.J Mol Microbiol Biotechnol 2017;27:11-21
Source: Journal of Molecular Microbiology and Biotechnology - Category: Microbiology Source Type: research