Identification of species-specific microsatellite markers in three Siniperca species by RNA-Seq

In this study, a total of 991 SSR primer pairs were identified in 8923 SSR-containing sequences (transcripts >1 kb) by RNA-Seq. Then, 566 loci were randomly selected for primer synthesis and 394 of which were successfully amplified in S. chuatsi. All the non-polymorphic loci (308) were evaluated among three Siniperca species, and 18 species-specific microsatellite markers were finally validated among three Siniperca species. These species-specific microsatellite markers would not only easily discriminate these three Siniperca species at juvenile stage, but also effectively monitor the statuses of hybridization and introgression for germplasm resources protection.

Source: Biochemical Systematics and Ecology - November 16, 2016 Category: Biochemistry Source Type: research