Measuring the Impact of Hydrocarbons on Rates of Nitrogen Fixation

We describe two methods to measure the bulk N2 fixation by the entire microcosm: the acetylene reduction assay and the analysis of bulk N isotope ratios following incubations with 15N2. Also, we present a method to determine N2 fixation at cellular level, based on nanoSIMS analysis of individual cells from microcosms incubated with 15N2.
Source: Springer protocols feed by Microbiology - Category: Microbiology Source Type: news