Detection of borrelia genomospecies 2 in ixodes spinipalpis ticks collected from a rabbit in canada.

DETECTION OF BORRELIA GENOMOSPECIES 2 IN IXODES SPINIPALPIS TICKS COLLECTED FROM A RABBIT IN CANADA. J Parasitol. 2016 Nov 11; Authors: Scott JD, Clark KL, Foley JE, Anderson JF, Durden LA, Manord JM, Smith ML Abstract Lyme disease is a serious health problem with many patients requiring in-depth clinical assessment and extended treatment. In the present study, we provide the first records of the western blacklegged tick, Ixodes pacificus, and Ixodes spinipalpis parasitizing eastern cottontails, Sylvilagus floridanus. We also documented a triple co-infestation of 3 tick species (Ixodes angustus, I. pacificus, I. spinipalpis) feeding on an eastern cottontail. Notably, we discovered a unique member of the Lyme disease bacterium, Borrelia burgdorferi sensu lato (s.l.) in Canada. Ixodes spinipalpis ticks, which were collected from an eastern cottontail on Vancouver Island, British Columbia (B.C.), were positive for B. burgdorferi s.l. Using PCR amplification on the tick extracts and DNA sequencing on the borrelial amplicons, we detected Borrelia genomospecies 2, a novel subgroup of the B. burgdorferi s.l. complex. Based on 416 nucleotides of the flagellin B (flaB) gene, our amplicons are identical to the Borrelia genomospecies 2 type strain CA28. Borrelia genomospecies 2 is closely related genetically to other B. burgdorferi s.l. genospecies, namely B. americana, B. andersonii, and B. burgdorferi sensu stricto (s.s.) that cause Lyme dise...
Source: The Journal of Parasitology - Category: Parasitology Authors: Tags: J Parasitol Source Type: research