Intra tracheal instillation of BMSC-cm attenuate acute rejection in rat lung allotransplantation

The aim of this study was to investigate the effect of secretome from bone marrow derived stem cells (BMSC) on acute lung rejection in rat lung allotransplantation model. Lung allotransplantation was performed from male BN donor to male Fisher F344 rats. Rat BMSC were grown in serum free condition and the supernatant bone marrow stem cells derived conditioned media (BMSC-cm) was collected. Recipients were instilled BMSC-cm intra tracheal every day for 5 days. Group A (n=5) received control media and low dose CsA (2.5 mg/kg bw ip) and group B (n=5) BMSC-cm and a low dose CsA, for 5 days post-transplant. Graft function was assessed by blood gas only from the graft, and tissue was sampled for histological grading of rejection and measurement of wet/dry ratio. All grafts in group B showed improved gas exchange 95.5±63.0 vs 42.7±18.07 mean PaO2 mmHg at day 5. Histological examination revealed moderate to severe rejection in all animals in group A (AIII B1) in contrast to improved histology in group B (IA B0). Moreover the wet/dry ratiowas also reduced in group B 7.18±0.4 compared to 10.4± 1.9 in group A. BMSC secretome is a promising novel method for localized cell based pharmotherapeutic approach for acute rejection in rat lung allograft model.
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: 8.2 Transplantation Source Type: research