A single and two step isomerization process for D-Tagatose and L-Ribose bioproduction using L-arabinose isomerase and D-lyxose isomerase

Publication date: Available online 9 November 2016 Source:Enzyme and Microbial Technology Author(s): Manisha J. Patel, Rekha C. Akhani, Arti T. Patel, Samir R. Dedania, Darshan H. Patel L-ribose and D-tagatose are biochemically synthesized using sugar isomerases. The L-arabinose isomerase gene from Shigella flexneri (Sf-AI) was cloned and expressed in Escherichia coli BL-21. Sf-AI was applied for the bioproduction of D-tagatose from D-galactose. L-ribose synthesis was performed by two step isomerization using Sf-AI and D-lyxose/ribose isomerase from Cohnella laevoribosii. The overall 22.3% and 25% conversion rate were observed for D-tagatose and L-ribose production from D-galactose and L-arabinose respectively. In the present manuscript, synthesis of rare sugars from naturally available sugars is discussed along with the biochemical characterization of Sf-AI and its efficiency.
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research