Endocytic Transport of Polyplex and Lipoplex siRNA Vectors in HeLa Cells

ConclusionsMP, CvME and FME, but not the acidified clathrin-mediated pathway, lead to effective gene silencing by PEI/siRNA polyplexes. Lipoplexes, in contrast, deliver siRNA primarily by direct fusion of the liposomal and cellular membranes. These results provide a new understanding of the mechanisms of siRNA delivery materials in HeLa cells and may aid in design of more effective RNAi strategies.
Source: Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research