PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas.

PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas.
Monoclon Antib Immunodiagn Immunother. 2016 Oct;35(5):263-266
Authors: Kaneko MK, Honma R, Ogasawara S, Fujii Y, Nakamura T, Saidoh N, Takagi M, Kagawa Y, Konnai S, Kato Y
Abstract
Podoplanin, a type I transmembrane protein, is expressed in lymphatic endothelial cells. Although we previously developed an anticanine podoplanin monoclonal antibody (mAb), PMab-38, immunohistochemistry (IHC) showed that it did not react with canine lymphatic endothelial cells. Here, we determined whether PMab-38 recognizes canine podoplanin of squamous cell carcinomas (SCCs) and clarified its epitope. In IHC, PMab-38 reacted with 83% of SCCs (15/18 cases). Flow cytometry showed that the epitope of PMab-38 was different from that of the platelet aggregation-stimulating domain of the N-terminus, which was detected by almost all antipodoplanin mAbs such as D2-40 or NZ-1. PMab-38 is expected to be useful for investigating the function of podoplanin in canine tumors.
PMID: 27788030 [PubMed - in process]

Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 29, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research