The suppressive effects of aluminum chloride on the osteoblasts function

Publication date: December 2016 Source:Environmental Toxicology and Pharmacology, Volume 48 Author(s): Yanzhu Zhu, Feibo Xu, Xijun Yan, Liguang Miao, Haitao Li, Chongwei Hu, Zhongying Wang, Shizhen Lian, Zhuo Feng, Yanfei Li Aluminum (Al) exposure impairs bone formation, and bone formation is mediated by the osteoblasts. But effects of Al on the osteoblasts function remain elusive. The osteoblasts were exposed to 0, 0.0252, 0.126, 0.252mg/mL AlCl3·6H2O for 24h. The osteoblasts viability, TGF-β1, BMP-2, IGF-I and Cbfα1 mRNA expressions, and GSH-Px and SOD activities, ROS concentration were determined. The osteoblasts ultrastructural features were also observed. The results showed that AlCl3 suppressed the osteoblasts viability, TGF-β1, BMP-2, IGF-I and Cbfα1 mRNA expressions, GSH-Px and SOD activities, and elevated ROS concentration compared with the CG. The ultrastructural features of osteoblasts in the HG showed mitochondrial swelling, foam-like structure, uneven distribution of chromatin, incomplete cell membrane and cytoplasm spillover compared with the CG. It indicates that AlCl3 inhibits osteoblasts viability, growth regulation factors mRNA expressions, anti-oxidative function, and damaged the osteoblasts histology structure, impairing the osteoblasts function. Graphical abstract
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research