Comprehensive optimization of LC –MS metabolomics methods using design of experiments (COLMeD)

ConclusionsThe COLMeD process elucidated response tradeoffs, facilitating improved chromatography and MS response without compromising separation of isobars. COLMeD is efficient, requiring no more than 20 injections in a given DoE round, and flexible, capable of class-specific optimization as demonstrated through acylcarnitine optimization within the QqQ method.

http://link.springer.com/10.1007/s11306-016-1132-4

Source: Metabolomics - October 23, 2016 Category: Biology Source Type: research

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