Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction

Interphase cytogenetics using archival tissue samples is a straightforward approach to obtain“cytogenetic information” from nuclei of solid tissue samples. It is the major tool to investigate specific numerical chromosomal aberrations, chromosomal translocations, amplification of oncogenes, or deletion of tumor suppressor genes in archival tissue samples on a single-cell level, as no metaphase spreads can be obtained, obviously. Here a collection of protocols for archival sectioned and mounted material as well as extracted nuclei (from formalin-fixed/paraffin-embedded or cryofixed tissues) is presented.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news