FISH in Uncultivated Amniocytes

In prenatal diagnostics, interphase FISH (iFISH) may be applied for the detection of certain numeric chromosomal aneuploidies. iFISH in uncultivated amniocytes can be performed from 12 weeks of gestation to the third trimester. Alternatively, the described aneuploidy screening set here is also suitable on short-term or uncultivated chorionic villus sampling (CVS) without metaphases as a fast screening test for the most common aneuploidies. Also this test helps to distinguish between culture-induced or real fetal triploidy or tetraploidy (mosaicism), which is found from time to time in the parallel cell culture; in this setting native cells are evaluated and they reflect the real fetal status. Commercially available chromosome-specific DNA probes for chromosomes 13, 18, 21, X, and Y are routinely applied for this. Thus, trisomies of chromosomes 13, 18, and 21, gonosomal aberrations, as well as tri- or tetraploidy can be identified. False-positive results are possible, but rare.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news