Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Bone Marrow, Chorion, Amniocytes, and Fibroblasts

Peripheral blood, amniocytic fluid, chorion, and fibroblasts are the most frequently used tissues for chromosome studies. All four of them are relatively easy to obtain and can simply be brought into short-term culture, and metaphase spreads can be prepared within rather short time. Such metaphase spreads, as well as the huge amounts of previously superfluous interphase nuclei in cytogenetic preparations, are a material very well suited for FISH analyses. All kinds of FISH probes can be used to analyze metaphase spreads, while the interphase nuclei actually can be analyzed for routine purposes only by satellite and locus-specific probes. As quality and quantity of metaphases are important in research and routine diagnostics, the preparation procedure of the chromosomes is outlined in more detail. Moreover, a standard two-color FISH approach including the possibility to enhance the DAPI-banding pattern by BrdU during cultivation is described here.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news