The Standard FISH Procedure

Molecular cytogenetics originally comprised of two basic approaches: fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS). Nowadays FISH is the one routine approach still used in research and routine molecular cytogenetics field. Here the basic protocol in how to do FISH using commercial and/or homemade DNA probes is described. For a protocol for FISH on tissue sections, please refer to chapter by Thomas Liehr“
Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction
.” Besides direct-labeled probes, also indirect-labeled probes can be used, and both variants are outlined here.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news