Microscopy and Imaging

Microscopy is an integral part of fluorescence in situ hybridization (FISH) and related techniques. In combination with imaging technologies, microscopy has become a basis for a variety of FISH-based approaches to qualitative and quantitative molecular cytogenetic analysis of nucleic acids in situ. Here, these basic components of FISH—microscopy and imaging—are discussed. To avoid reproducing numerous textbooks dedicated to the fundamentals of microscopy (including the previous edition of this chapter in 2009) and manufacturer’s brochures about commercially available imaging systems, the basic aspects of microscopy and imaging are reviewed in the light of the latest FISH-based molecular cytogenetic developments.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news