MYPT1 isoforms expressed in HEK293T cells are differentially phosphorylated after GTP γS treatment.

This study was designed to determine if Rho kinase differentially phosphorylates MYPT1 isoforms. In HEK293T cells expressing each of the four MYPT1 isoforms, we could not detect a change in Thr853 MYPT1 phosphorylation following GTPγS treatment. However, there is differential phosphorylation of MYPT1 isoforms at Thr696; GTPγS treatment increases MYPT1 phosphorylation for the CI+LZ- and CI-LZ- MYPT1 isoforms, but not the CI+LZ+ or CI-LZ+ MYPT1 isoforms. These data could suggest that in smooth muscle Rho kinase differentially phosphorylates MYPT1 isoforms.
PMID: 27725371 [PubMed - in process]

Source: Journal of Smooth Muscle Research - October 14, 2016 Category: Research Tags: J Smooth Muscle Res Source Type: research