Burkholderia pseudomallei resistance to antibiotics in biofilm induced conditions is related to efflux pumps.

Burkholderia pseudomallei resistance to antibiotics in biofilm induced conditions is related to efflux pumps.
J Med Microbiol. 2016 Sep 30;:
Authors: Sirijant N, Sermswan RW, Wongratanacheewin S
Abstract
Burkholderia pseudomallei, the causative agent of melioidosis, has been found to increase its resistance to antibiotics when growing as a biofilm. The resistances are related to several mechanisms. One of the possible mechanisms is the efflux pump. Using bioinformatics analysis, it was found that BPSL1661, BPSL1664 and BPSL1665 were orthologous genes of the efflux transporter encoding genes for biofilm related antibiotic resistance PA1874-PA1877 genes in Pseudomonas aeruginosa strain PAO1. Expressions of selected encoding genes for the efflux transporter system during biofilm formation were investigated. Real-time RT-PCR expression of amrB, cytoplasmic membrane protein of AmrAB-OprA efflux transporter encoding gene, was slightly increased while BPSL1665 were significantly increased during growing of bacteria in biofilm formations. Minimum biofilm inhibition concentration (MBIC) and minimum biofilm eradication concentration (MBEC) of ceftazidime (CTZ), doxycycline (DOX) and imipenem (IMN) were found 2- to 1024-times increased when compared to their minimum inhibition concentrations (MIC) of planktonic cells. Inhibition of the efflux transporter by adding phenylalanine arginine beta-napthylamide (PAβN), a universal efflux inhibitor, d...

Source: Journal of Medical Microbiology - September 29, 2016 Category: Microbiology Authors: Sirijant N, Sermswan RW, Wongratanacheewin S Tags: J Med Microbiol Source Type: research