Antimicrobial Susceptibility of Microorganisms Isolated from Periapical Periodontitis Lesions.

Antimicrobial Susceptibility of Microorganisms Isolated from Periapical Periodontitis Lesions. Bull Tokyo Dent Coll. 2016;57(3):133-42 Authors: Narita M, Shibahara T, Takano N, Fujii R, Okuda K, Ishihara K Abstract Periapical periodontitis usually results from microbial infection, with these microorganisms occasionally migrating to the root canal, which can lead to further, potentially life-threatening, complications. Here, the susceptibility of 27 bacterial strains to various antimicrobial agents was evaluated. These strains comprised 13 species; 16 of the strains were clinical isolates from periapical lesions. Each strain was inoculated onto blood agar plates containing one of the antimicrobial agents. The plates were incubated anaerobically at 37°C for 96 hr and the minimal inhibitory concentrations (MICs) determined. Ten strains required an MIC of 32 μg/ml or greater for amoxicillin, 6 for cefmetazole, and 5 for cefcapene among β-lactam antibiotics; 8 strains required an MIC of 32 μg/ml or greater for clindamycin, 4 for azithromycin, and 11 for clarithromycin among macrolide antibiotics; 3 strains required an MIC of 32 μg/ml or greater for ciprofloxacin and 2 for ofloxacin among fluoroquinolones. The effect of cefcapene on 5 strains was evaluated after biofilm formation to investigate the relationship between biofilm formation and susceptibility. All strains showed a decrease in susceptibility after biofilm formation. The re...
Source: The Bulletin of Tokyo Dental College - Category: Dentistry Authors: Tags: Bull Tokyo Dent Coll Source Type: research