Heavy enzymes-experimental and computational insights in enzyme dynamics.

Heavy enzymes-experimental and computational insights in enzyme dynamics.
Curr Opin Chem Biol. 2014 Apr 4;21C:11-18
Authors: Swiderek K, Javier Ruiz-Pernía J, Moliner V, Tuñón I
Abstract
The role of protein motions in the chemical step of enzyme-catalyzed reactions is the subject of an open debate in the scientific literature. The systematic use of isotopically substituted enzymes has been revealed as a useful tool to quantify the role of these motions. According to the Born-Oppenheimer approximation, changing the mass of the protein does not change the forces acting on the system but alters the frequencies of the protein motions, which in turn can affect the rate constant. Experimental and theoretical studies carried out in this field are presented in this article and discussed in the framework of Transition State Theory.
PMID: 24709164 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - April 4, 2014 Category: Biochemistry Authors: Swiderek K, Javier Ruiz-Pernía J, Moliner V, Tuñón I Tags: Curr Opin Chem Biol Source Type: research