Development of Antibiotic-Free Selection System for Safer DNA Vaccination

The use of antibiotic-resistance markers in DNA vacciness is discouraged by regulatory agencies due to various theoretical safety concerns. This chapter presents methodologies for the design and cloning of synthetic antigen genes into RNA-OUT encoding antibiotic-free DNA vaccines vectors that are additionally optimized to improve protein expression, and immunogenicity, compared to alternative kanamycin-resistant vectors.
Source: Springer protocols feed by Immunology - Category: Allergy & Immunology Source Type: news